Journal: Biomedicines

Article Title: In Vivo Evaluation of ECP Peptide Analogues for the Treatment of Acinetobacter baumannii Infection

doi: 10.3390/biomedicines10020386

Figure Lengend Snippet: Evaluation of CFUs, TNF- α and LPS in the 2nd efficacy assay using 20 mg/kg of peptide treatment. ( a ) The average CFUs in mice organs (spleen and lung). ( b ) The average concentration of TNF-α in mice serum. ( c ) The average concentration of LPS in mice serum was quantified by ELISA as described in the methodology. All the animals from the colistin group survived until Day 3, so no data for organs and serum analysis can be shown at Day 0. Regarding the vehicle group, no animals survived after Day 0, so no data can be shown at Day 3. Each animal had been inoculated 10 8 CFU/Kg A. baumannii with 5% mucin before treatment. A single treatment was administrated 2 h after the bacteria inoculation. b,c were obtained after the correction of OD 450 with baseline mice, which are neither infected nor peptide treated (only injected at Day 0 with HBS). Mann—Whitney test was used for the comparison between treatments and vehicle except for the additional comparison between ECPep-D and colistin in c at day 3 ( ** p < 0.01, * p < 0.05, # p < 0.1).

Article Snippet: Mouse TNF-α ELISA Kit (MBS175787, MyBioSource, San Diego, CA, USA) and Mouse LPS ELISA Kit (MBS7700668, MyBioSource, San Diego, CA, USA) were from bioNova científica s.l. (Madrid, Spain) Human TNF-α ELISA set was from BD Biosciences (555212, BD OptEIA TM , San Jose, CA, USA).

Techniques: Concentration Assay, Enzyme-linked Immunosorbent Assay, Bacteria, Infection, Injection, MANN-WHITNEY, Comparison